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Image Search Results
Journal: Molecular Therapy. Nucleic Acids
Article Title: Nanoparticle delivery of VEGF-B mRNA promotes T cell infiltration within tumor and triggers robust antitumor immunity
doi: 10.1016/j.omtn.2025.102620
Figure Lengend Snippet: Characterization of VEGF-B mRNA modifications and mRNA-LNPs (A and B) IP-western blot analysis of VEGF-B protein expression in HEK293T and B16F10 cells after transfection of VEGF-B mRNA with different modifications and tailing method. Non, non-modified; m1Ψ, m1Ψ-modified and EPAP tailing; Ψ1, Ψ-modified and EPAP tailing; Ψ2, Ψ-modified and co-transcriptionally tailing. (C) Transmission electron microscopy observation of VEGF mRNA-LNP; scale bars, 200 μm. (D) Size distribution of VEGF mRNA-LNP and control mRNA-LNP detected by Malvern dynamic light scattering Zetasizer. (E) VEGF-B protein secretion levels in B16F10 cells transfected with VEGF-B mRNA-LNP, detected by ELISA. Cells in a six-well plate were transfected with 2 μg of the modified mRNA per well. Data are presented as the median ± standard error of the mean (SEM). Significance was determined with ordinary one-way ANOVA in (E). ∗∗∗∗ p < 0.0001.
Article Snippet: The concentration of VEGF-B protein was quantified through ELISA with the
Techniques: Western Blot, Expressing, Transfection, Modification, Transmission Assay, Electron Microscopy, Control, Enzyme-linked Immunosorbent Assay